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Tosoh Bioscience offers a comprehensive line of products including TSKgel®, TOYOPEARL®, and Ca++Pure-HA™ chromatography media, SkillPak™ pre-packed process development columns, TSKgel U/HPLC columns, the LenS3™ multi-angle light scattering detector, and a unique EcoSEC® GPC System for polymer characterization. These products satisfy the needs of the biochemist and the biotechnologist involved in analyzing, isolating and purifying proteins, peptides, enzymes, oligonucleotides, antibiotics, and small biomolecules.
A highly selective collection of process media for oligonucleotide purification is available from Tosoh Bioscience, which includes TSKgel SuperQ-5PW (20) and (30) resins as well as TOYOPEARL GigaCap® Q-650S and SuperQ-650S. TSKgel SuperQ-5PW (20) is the market leader for oligonucleotide purification. This resin has high chemical and mechanical stability, allowing cleaning-in-place (CIP) and high flow rates. It is best used for capture of oligonucleotides from crude harvest in a one-step purification process. Chromatographic efficiency, peak width and resolution are improved because of the smaller, 20 µm, particle size.
Simple and effective methods for the purification, as well as the molecular weight analysis, of DMT-on oligonucleotides and DMT-off oligonucleotide therapeutics has been developed by Tosoh Bioscience. Several TOYOPEARL hydrophobic interaction chromatography (HIC) resins (PPG-600M, Phenyl-650M, Butyl-650M, and Hexyl-650C) can be used for DMT-on oligonucleotide purification. On-column DMT cleavage using these HIC resins provide >99% purity and recovery of DMT-protected oligonucleotides. The anion exchange resin, TSKgel SuperQ-5PW (20), is the solution for the purification of charged DMT-off oligonucleotides, easily resolving N+1 and N-1 impurities. When used in a single step purification, TSKgel SuperQ-5PW (20) results in >97% purity of DMT-off oligonucleotides. Accurate molecular weight profiling of oligonucleotides can be achieved using a TSKgel UP-SW2000 size exclusion chromatography HPLC column coupled with the LenS3 light scattering detector.
Tosoh Bioscience has operations in Japan, Germany, China, Singapore, India and the United States. Products are manufactured by Tosoh Corporation in Japan, and our office in the United States is located in King of Prussia, PA.