Day Two (14th May 2025)Wednesday, 14 May 2025 - CET/CEST (Cent Europe Summer, GMT+2)

To drive down the cost of goods, biomanufacturers are striving towards maximizing facility utilization, increasing process efficiency, and maintaining a robust supply chain—all without compromising the quality of the final product.

Designed specifically for the hydration of powdered media, feeds, or buffers, the Oceo Rover single-use system delivers consistent solutions on demand in less than half the time of conventional processes. In this session, you will learn how to transform your bioprocessing operations with the first alternative system for media feed and buffer preparation. The Oceo Rover will be demonstrated together with chemically defined media from FUJFILM Irvine Scientific to help you achieve greater efficiencies.

Discover how the Oceo Rover:

• Delivers bioprocessing solutions for both process development and manufacturing, in the upstream and downstream environment, and across multiple modalities
• Hydrates and sterilizes media, feeds, or buffers as a single-unit operation
• Provides repeatable and scalable results using prepacked, single-use cartridges with our chemically defined media portfolio
• Improves environmental, health, and safety considerations and eliminates the risk of cross-contamination via a self-contained system
• Features access to world-class support to help you identify the ideal hydration protocol and optimize your workflows

Current downstream bioprocessing methods rely heavily on time-consuming off-line analyses for quality monitoring, such as product titer determination and contaminant detection. These traditional approaches limits process efficiency and real-time decision-making. While various attempts have been made to implement in-line and on-line measurement technologies, these efforts have seen limited success. In this presentation, we introduce AugaOne, an innovative in-line sensor system specifically designed to enhance downstream bioprocessing. Built on a nanoplasmonic sensing technology platform, AugaOne enables real-time monitoring of critical quality attributes and key process parameters, ensuring optimal process control. Its modular design allows seamless integration into existing purification and separation workflows, driving process intensification and improving overall efficiency. By enabling continuous monitoring and control, AugaOne paves the way for more robust and streamlined downstream biomanufacturing.

• Limitations of viral vectors & need for alternatives

• Emerging delivery tech:

• LNPs, Exosomes

• How novel approaches/systems can aid in:

• Safety, reduced immunogenicity

Cell culture media are complex mixture that are critical to ensure the robust production of biopharmaceutical products. Cell culture media quality is however dependent of multiple parameters such as raw material quality and preparation process. While protocols are in place at the biopharmaceutical site as well as at the raw material manufacturer to ensure reproducible preparation, there is to date little technology available to verify that the composition of the medium produce is as expected. This gap lies within the fact that media composition is very heterogenous comprising compounds in very large ranges of concentrations from nM to M and very different chemical properties including metals, salts, organic compounds as well as polymers and proteins. This study shows how we can come closer to closing this gap with an on-line fluorescence-based method for the monitoring of cell culture media preparation. We will show the characterization of the method including sensitivity and scale up as well as the challenges we face for implementation in commercial facilities.

Galactose and Mannose are two important carbohydrates that are widely used as cell culture media supplements for recombinant protein production (Innovator protein therapeutics, Biosimilars, Biobetters etc.) and other applications. Galactose not only plays important role in protein Glycoform modulation but also aids in Cellular energy production, Synthesis of Glycoproteins and Glycolipids and Energy storage. Similarly, Mannose plays important role in Glycoform modulation, energy production and signal transduction. Purity and quality are two very important aspects that must be considered when choosing the cell culture media supplements. Contamination of cell culture media supplements with heavy metals can significantly impact cell growth and function. Metals/Elemental Impurities such as copper, iron, lead, manganese, zinc, and cadmium can enter cell culture media through various sources, including the raw materials used to manufacture the media. Therefore, it is of paramount importance to use highly pure, well characterized, and low metal containing supplements (including Galactose and Mannose) in cell culture applications. In this presentation, I will present case studies demonstrating the importance of Galactose and Mannose supplementation in Cell Culture and talk about Pfanstiehl high purity, low endotoxin and low metal Galactose and Mannose offerings.

• Cross-team collaboration: Integrate sustainability principles across the downstream spectrum
• Minimizing biological load in waste streams: Strategies and best practices
• The Role of Innovation: Developing new technologies and approaches for a greener future
• Process intensification and its impact on sustainability
• The importance of facility design for achieving sustainable manufacturing goals
• How can outsourcing of process liquids be a more sustainable choice?
• What additional strategies can companies adopt to make their buffer-related operations more sustainable?

Explore groundbreaking research, meet the poster presenters and BPI Editors!

*For your poster to be entered into the poster competition, please submit your abstract by 31st March 2025 and PDF poster by 14th April*