Main Conference - Day 1 Keynote Sessions (Apr 27)Tuesday, 27 April 2027

This presentation explores analytical strategies for characterizing lipid-conjugated oligonucleotide therapeutics. It examines both denaturing and nondenaturing chromatographic approaches, highlighting trade-offs between single-strand selectivity and dual-strand monitoring. The talk also discusses the use of HILIC for separating lipid conjugates and the challenges posed by solubility, column chemistry, and duplex stability. Emphasis is placed on optimizing column selection, mobile phase composition, and sample preparation to achieve reliable purity assessment.