Add-on this pre-conference training course to your main conference registration package for an additional fee and gain a comprehensive overview of antibody engineering in an easy-to-follow classroom setting to help you prepare for the main conference program.

• Training course registration begins at 8:00am.
• Break Schedule:
  • AM Break: 10:30-11:00;
  • Lunch: 12:30-1:30;
  • PM break: 3:00-3:30

TRAINING COURSE OVERVIEW

Today’s wealth of knowledge of protein structures will be reviewed along with the genetics of diversity generation of antibodies, to give insights into the best strategies for improving protein function. There is particular emphasis on the choice of a functional assay to effectively monitor the changes in a desired property, and the use of functional enrichment steps where a library approach is employed. Not only is amino acid sequence amenable to engineering, but glycan structures and other modifications may also be engineered. The course will focus on the engineering and enhancement of antibodies and antibody-like scaffolds. Examples will include work on antibody fragment affinity improvement by 100-fold to low pM affinity. Also, the engineering of bispecific antibodies by diverse approaches and the adaptation to generate Chimeric Antibody Receptor (CAR) constructs will be discussed. Expression platforms for producing antibodies for testing and for manufacture will also be covered. A background in biochemistry and molecular biology is useful, as the course is designed to progress rapidly from simple to advanced concepts.

INSTRUCTOR

David Bramhill, Ph.D., Founder, Bramhill Biological Consulting, LLC and Research Corporation Technologies

COURSE AGENDA

• Functions amenable to engineering: affinity, specificity, stability, solubility, immunogenicity
• The measure of success: functional assays
• Engineering by design
• Engineering by random mutation
• Designed libraries
• Display technologies
• Improving manufacturing by protein engineering methods
• Glycosylation engineering – function and homogeneity
• Other protein modifications
• Immunogenicity engineering
• Bispecific antibodies
• Antibody-drug conjugates (ADCs)
• CAR-T strategies
• Expression of antibodies and fragments for discovery and testing
• Manufacturing platforms for antibodies and fragments